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KMID : 0357319810160010049
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Journal of the Korean Society for Microbiology
1981 Volume.16 No. 1 p.49 ~ p.55
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Modulation of Immune Response by Cimetidine
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河大有/Ha, Tai You
李憲九/宋陽根/Lee, Hern-Ku/Song, Yang-Keun
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Abstract
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Recent studies have demonstrated that histamine could have a modulatory influence on the immune response in vitro and in vivo. However, the effect of histamine on immune response in mice has not been extensively analyzed.
In the present study the regulatory effects of cimetidine, a histamine-2-receptor antagonist(H2 blocker) and histamine on the immune response to sheep red blood cells (SRBC) were evaluated in mice. Mice pretreated with daily intraperitoneal injection of varying concentrations of cimetidine for 14 days were immunized intraperitoneally with various concentrations of SRBC(108, 10´, and 108 cells) and challenged 4 days post immunization. The cellular immune response was determined by measuring the footpad swelling reaction. Footpad swelling reaction of each. mouse was measured at 3hr (Arthus) reaction) and 24 or 48 hr .(delayed´ reactions) after challenge. The humoral immune response was determined by measuring hemagglutinins to SRBC.´ Histamine in varying concentrations (10-1. 10´3 and .10-BM (was added in SRBC suspension. at the time of antigen challenge into footpad, and 24-hr delayed type hypersensitivity (DTH) was measured.
Cimetidine in varying concentrations (10, 50, 250,1250 and 6250,ug) enhanced 24-hr DTH and this enhancement of DTH was more pronounced at 250,ug of cimetidine. However, there were no significant differences between the cimetidine-pretreated groups and controls in Arthus reaction and hemagglutinin titers. 1
Histamine suppressed the DTH in the dose=dependent fashion. This suppression was more pronounced at lower concentration of immunizing antigen (10´ and 108 SRBC). However, histamine did not diminish the DTH at higher concentration of antigen (108 SRBC).
These results present the evidences which strongly suggest that cimetidine enhances the cell-mediated immune response but not significantly influences the humoral immune response and that exogenous and. endogenous histamine is involved in the modulation of cellular immune response as well as immediate hypersensitivity.
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KEYWORD
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